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Effect of probiotics use on fatty liver and metabolic endotoxemia, in rats fed with high fat diet

 2018 EuDAP Report / Posted 7 months ago by admin / 47 views

Non alcoholic fatty liver disease (NAFLD) is a complex and multifactorial disease,
it’s pathogenesis is not fully understood. The pathogenesis of disease is involves a
complex process, genetic polymorphism, enviromental factors and change of
intestinal microbiota. Lifestyle changes and physical activity are primary goals in the
treatment. In this study, the effect of probiotic use on the fatty liver and metabolic
endotoxemia in rats fed with high fat diet was invsetigated. Sprague Dawley rats
(n=24) were used in this study and animals were divided into 3 groups. The control
group received standard purified feed, the other two groups respectively, receieved
high fat feed+plasebo (1 drop of NaCl), high fat feed+probiotic Lactobasillus r. GG
(1×10¹⁰ cfu/ 1 drop) for 16 weeks. At the end of the period, blood samples were
taken from rats, mesenteric, perirenal, gonadal fat tissue levels were measured and
the liver was removed for the histopathology. When the serum AST levels of groups
were compared, statistical difference was found as significant, the highest level was
found in high fat feed+plasebo group. The difference between the mean serum ALT
levels of groups was found be significant and lowest ALT level was found in the
control, the highest level high fat feed+probiotic group. There was no statistical
difference between groups, cholesterol, TNF-α, CRP and LPS level (p>0.05). There
was no correlation between LPS with AST, ALT, cholesterol and CRP levels
(p>0.05). The difference between in gropups, mean of liver weight, perirenal and
mesenteric fat content was significant (p<0.05). At the histopathological examination
of the groups revealed steatosis negative in control group, 62.5 % of in high fat
feed+plasebo group and 12.5% hepatosteatosis + hepatocellular balloning in high fat
feed+probiotic group (1 animal). As a result, the protective effect of supplementation
with probiotics on high fat diet induced fatty liver was demonstrated as
histopathologically but its effects on liver enzymes, inflamatory markers and
metabolic endotoxemia has not been fully demonstrated. In order to recommend of
the use probiotics in NAFLD, more researches are needed in terms of both dose and
strain.
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Key Words: Probiotics, endotoxemia, non alcoholic fatty liver, high fat diet.

  • National Dietetic Association: TURKEY - Turkish Dietetic Association, Türkiye Diyetisyenler Dernegi
  • EFAD Education Associate Member: none or other than from the list
  • EuDAP key objectives: Objective 3. Use dietitians as educators and experts in the community and clinical settings
  • Target audience: Health professionals, Special Groups
  • Type: Document, Research
  • Read more (link): http://dspace.baskent.edu.tr/bitstream/handle/11727/2976/10225834.pdf?sequence=1&isAllowed=y
Contact details

Sevan Çetin, Izmir Democracy University Health Sciences Faculty Department of Nutrition And DieteticsTurkey Show Phone Number ***** https://orcid.org/0000-0002-3451-9834

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